´╗┐After washing to remove any unbound antibody-enzyme reagent, a substrate solution was added to the wells and color was developed in proportion to the amount of KIM-1 bounded in the initial step

´╗┐After washing to remove any unbound antibody-enzyme reagent, a substrate solution was added to the wells and color was developed in proportion to the amount of KIM-1 bounded in the initial step. (Perkin Elmer, USA). All ELISA results were analyzed with WorkOut 2.5 software and indicated as the imply concentration in nanograms protein/ml. KIM-1 dedication […]