Cell culture supernatant from cells transfected with plasmid was loaded onto proteins A affinity column transiently

Cell culture supernatant from cells transfected with plasmid was loaded onto proteins A affinity column transiently. dependant on cryo-electron microscopy. We characterized antibody-binding specificities and cell-sorting capabilities from the biotinylated probes also. Altogether, structure-based design combined to effective purification and biotinylation processes can enable streamlined advancement of SARS-CoV-2 spike-ectodomain probes thus. Keywords: antibody, biotinylated probe, […]

Generation of monoclonal antibodies against TGEV RdRp Murine mAbs specific for RdRp were obtained by standard procedures (Harlow and Lane, 1988)

Generation of monoclonal antibodies against TGEV RdRp Murine mAbs specific for RdRp were obtained by standard procedures (Harlow and Lane, 1988). 62-amino acid region of the N-terminal domain, suggesting that this region may constitute an immunodominant domain. The availability of TGEV RdRp mAbs will be instrumental to study coronavirus replication and to analyze the function […]