Furthermore, the selectivity coefficient to each interfering compound could be determined as the ratio of the slope of the calibration plot for IgG and a given compound including HSA, BSA and Lyz (Fig. IgG is the only human immunoglobulin that can pass through the placenta, which exerts a vital part in the anti-infective immunity in newborns [4], [5], [6]. IgG is highly expressed in serum and accounts for 75C80% of all immunoglobulins in serum. Typically, it is widely distributed and easily penetrates the capillary wall. Moreover, it is also an important antibody existing outside the blood vessels [7]. The abnormal IgG level will induce certain disorders, like hyperthyroidism and systemic lupus erythematosus (SLE). COVID-19 pandemic outbreak is one of the most shocking scenes of the XXI century. A convenient way to detect the presence of the novel coronavirus is to use substrates (i.e. sensor material) that has been functionalized with recombinant viral antigens and can be used to bind to IgG because these antibodies remain in the blood longer [8]. IgG antibodies are produces during the onset of the infectious disease (around 2?weeks). Therefore, it is of critical importance to detect the content of IgG in the fields of quality monitoring, drug research and medical diagnosis [9], [10]. To date, various analytical techniques such as radial immunodiffusion, enzyme-linked immunoassays, immune nephelometry and fluorescent immunoassays, have been proposed in literature for the determination of IgG [11], [12], [13], [14], [15]. However, most of these techniques have some limitations such as expensive and cumbersome instruments, complicated pretreatment step, and time consuming analysis. Compared with these instrumental methods, the electrochemical measurement of IgG has the advantage of simplicity, high sensitivity, fast response and low detection limit [16], [17]. Extensive efforts have been made to electrochemically determine immunoglobulins, notably, the isotype of IgG which is dominant during low content detection in a majority of studies [18], [19]. Currently, many studies have depicted numerous diverse strategies in preparing the IgG-specific electrochemical sensors that can be used as the classic model analyte [20], [21], [22]. Generally, the electrochemical sensors based on nanomaterials can enhance sensor sensitivities through boosting the transducer electrochemical performances and using the biorecognition molecules to increase the sensing surface loading [23], [24], [25], [26]. Glassy carbon electrode (GCE) is the basis of a majority of electrochemical sensors, and conductive nanomaterials are Rabbit polyclonal to PPA1 then used to modify GCE for improving its sensitivity to target analytes [27], [28]. Many novel nanomaterials can be applied in modifying GCE, including MoS2 [29], [30], metal nanoparticles [31], [32], graphene quantum dot [33] and carbon black [34], [35]. MoS2 is a typical dichalcogenide, which attracts wide interests in numerous fields, such as hydrogen catalysis and Docetaxel (Taxotere) storage, capacitors, and electrochemical device [36], [37]. However, because the conductivity of MoS2 is still lower than that of carbon-based materials, little attention has been paid to the application of MoS2 as an electrode material for sensors [38]. Therefore, it is suggested to combine MoS2 with carbon-based materials to show a synergistic effect on the application of electrocatalysis [39]. Guochuang Huang et al. obtained a layered MoS2-Graphene composite modified electrode with good electrochemical performance, and it was able to detect paracetamol with high sensitivity and selectivity by L-cysteine assisted liquid phase method [40]. Tong Guo et al. designed a novel three-dimensional (3D) layered MoS2@graphene functionalized with N-GQDs composites, which was used as an enhanced electrochemical hydrogen evolution catalyst [41]. As Docetaxel (Taxotere) a result, it is reasonable to speculate that, integrating Docetaxel (Taxotere) the layer-structured MoS2 with nitrogen-doped graphene quantum dots (N-GQDs) also exerts same function in electrochemical sensors and electrocatalysis. Moreover, more and more importance has been attached to ionic liquids (IL) during sensor construction due Docetaxel (Taxotere) to optimized structures and properties, distinguishable anion/cation combination, high conductivity, remarkable biocompatibility, and good thermostability [42], [43]. Ionic liquid is an effective modifier to improve the sensing performance of electrode [44]. These results indicate that the use of ionic liquids can.